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VDAC1/Porin Polyclonal antibody

VDAC1/Porin Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

FC, IF, IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 55259-1-AP

Print datasheet

Synonyms

hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC1/Porin



经过测试的应用

Positive WB detected inRAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells
Positive IHC detected inhuman heart tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman liver cancer tissue
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VDAC1 Ready-To-Use IHC Kit. VDAC1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55259-1-AP targets VDAC1/Porin in WB, PLA, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, PLA, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, monkey, hamster, pig, canine, bovine
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name voltage-dependent anion channel 1
Synonyms hVDAC1, Plasmalemmal porin, Porin, PORIN 31 HL, Porin 31HL, Porin 31HM, VDAC, VDAC 1, VDAC1, VDAC1/Porin
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberNM_003374
Gene Symbol VDAC1
Gene ID (NCBI) 7416
RRIDAB_10837225
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP21796
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

实验方案

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
IHC protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
IF protocol for VDAC1/Porin antibody 55259-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar
monkeyWB

Nat Commun

An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.

Authors - Jingru Wang
humanWB

Nucleic Acids Res

The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.

Authors - Gui-Xin Peng
IHC

Mol Biol Evol

Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans

Authors - Ming Zhou
mouseWB

Redox Biol

Adiponectin/AdiopR1 signaling prevents mitochondrial dysfunction and oxidative injury after traumatic brain injury in a SIRT3 dependent manner.

Authors - Shenghao Zhang
humanWB

Autophagy

KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.

Authors - Guokai Yan