USP4 Monoclonal antibody

USP4 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse and More (2)

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1G11G8

Cat No : 66822-1-Ig

Print datasheet

Synonyms

Deubiquitinating enzyme 4, Ubiquitin thiolesterase 4, UNP, UnpEL, Unph, USP4



经过测试的应用

Positive WB detected inJurkat cells, NCI-H1299 cells, HEK-293 cells, RAW 264.7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, human liver cancer tissue
Positive FC detected inTHP-1 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:300
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66822-1-Ig targets USP4 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity Human, Mouse
Cited Reactivityhuman, mouse
Immunogen USP4 fusion protein Ag28297 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 4 (proto-oncogene)
Synonyms Deubiquitinating enzyme 4, Ubiquitin thiolesterase 4, UNP, UnpEL, Unph, USP4
Calculated Molecular Weight 963 aa, 109 kDa
Observed Molecular Weight 109 kDa
GenBank Accession NumberBC125131
Gene Symbol USP4
Gene ID (NCBI) 7375
RRIDAB_2882165
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13107
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP4, also named as UNP and UNPH, belongs to the peptidase C19 family and USP4 subfamily. USP4 is a deubiquitinating enzyme that links to mitogen-activated protein kinase signaling, pre-mRNA splicing, and control of p53 stability (PMID: 26455393). USP4 has 3 isoforms with the molecular mass of 36, 104 and 109 kDa. Recently, it has been reported that USP4 is a critical factor in promoting lung cancer stemness and potentially useful lung cancer prognosis marker (PMID: 32549341).

实验方案

Product Specific Protocols
WB protocol for USP4 antibody 66822-1-IgDownload protocol
IHC protocol for USP4 antibody 66822-1-IgDownload protocol
IF protocol for USP4 antibody 66822-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Death Dis

USP4 promotes the proliferation, migration, and invasion of esophageal squamous cell carcinoma by targeting TAK1

Authors - Hongdian Zhang
mouseWB

bioRxiv

The DNA helicase FANCJ (BRIP1) functions in Double Strand Break repair processing, but not crossover formation during Prophase I of meiosis in male mice

Authors - Tegan S Horan
humanWB,IF,IP

PLoS One

USP4 promotes the proliferation and glucose metabolism of gastric cancer cells by upregulating PKM2

Authors - Yuanyuan Chen