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USP18 Polyclonal antibody

USP18 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,ELISA

Conjugate

Unconjugated

Cat No : 12153-1-AP

Print datasheet

Synonyms

43 kDa ISG15 specific protease, hUBP43, ISG43, Ubl thiolesterase 18, UBP43, USP18

验证数据展示

  • WB analysis of MCF-7 using 12153-1-AP

    WB analysis of MCF-7 using 12153-1-AP

    WB result of USP18 antibody(12153-1-AP) with MCF-7 cells (Control and CRISPR USP18).

经过测试的应用

Positive WB detected inMCF-7

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12153-1-AP targets USP18 in WB,ELISA applications and shows reactivity with human samples.

Tested Applications WB,ELISA
Tested Reactivity human
Immunogen USP18 fusion protein Ag2785 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 18
Synonyms 43 kDa ISG15 specific protease, hUBP43, ISG43, Ubl thiolesterase 18, UBP43, USP18
Calculated Molecular Weight 372 aa, 43 kDa
Observed Molecular Weight 43 kDa, 70 kDa
GenBank Accession NumberBC014896
Gene Symbol USP18
Gene ID (NCBI) 11274
RRIDAB_2877830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UMW8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP18(UBP43) is a 43 kDa protein that is known as an ISG15-deconjugating enzyme and an inhibitor of type I IFN signaling independent of its enzyme activity. USP18 can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. It can be detected two bands(apparent MW of 38 and 35 kDa), the faster migrating one results from proteolytic processing in human primary hepatocytes(PMID:21779393). Sometimes the 70 kDa band is showed on the SDS-PAGE that it is considered as the complex of ISG15 and USP18(PMID: 14673145).