• Featured Product
  • KD/KO Validated

TRIM32 Polyclonal antibody

TRIM32 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10326-1-AP

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Synonyms

72 kDa Tat interacting protein, BBS11, HT2A, LGMD2H, TATIP, TRIM32, tripartite motif containing 32, Zinc finger protein HT2A



经过测试的应用

Positive WB detected inHEK-293 cells
Positive IHC detected inhuman lymphoma tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, mouse embryo tissue
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy TRIM32 Ready-To-Use IHC Kit. TRIM32 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10326-1-AP targets TRIM32 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen TRIM32 fusion protein Ag0401 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name tripartite motif-containing 32
Synonyms 72 kDa Tat interacting protein, BBS11, HT2A, LGMD2H, TATIP, TRIM32, tripartite motif containing 32, Zinc finger protein HT2A
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 65-72 kDa
GenBank Accession NumberBC003154
Gene Symbol TRIM32
Gene ID (NCBI) 22954
RRIDAB_2208005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13049
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TRIM32(Tripartite motif-containing protein 32) is a widely expressed ubiquitin ligase that is localized to the Z-line in skeletal muscle(PMID:19349376). It promotes PIAS4 ubiquitination and degradation, and by controlling PIAS4 stability, regulates UVB-induced keratinocyte apoptosis through induction of NFkappaB(PMID: 16816390).

实验方案

Product Specific Protocols
WB protocol for TRIM32 antibody 10326-1-APDownload protocol
IHC protocol for TRIM32 antibody 10326-1-APDownload protocol
IF protocol for TRIM32 antibody 10326-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Adv

An EMT-primary cilium-GLIS2 signaling axis regulates mammogenesis and claudin-low breast tumorigenesis.

Authors - Molly M Wilson
mouse,humanIHC

J Am Soc Nephrol

Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.

Authors - Sybille Koehler
mouseWB

Hum Mol Genet

Deficiency of the E3 ubiquitin ligase TRIM32 in mice leads to a myopathy with a neurogenic component.

Authors - Kudryashova Elena E
  • KO Validated
human,ratWB,IF,IP

FASEB J

Ubiquitin ligase TRIM32 promotes dendrite arborization by mediating degradation of the epigenetic factor CDYL.

Authors - Lei Liu
  • KD Validated
humanIF

J Cell Sci

TRIM32, but not its muscular dystrophy-associated mutant, positively regulates and is targeted to autophagic degradation by p62/SQSTM1.

Authors - Katrine Stange Overå
humanWB

iScience

A nonautophagic role of ATG5 in regulating cell growth by targeting c-Myc for proteasome-mediated degradation.

Authors - Sheng Li