CoraLite® Plus 488-conjugated TNF Alpha Monoclonal antibody

TNF Alpha Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (1)

Applications

IF and More (1)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

7B8A11

Cat No : CL488-60291

Print datasheet

Synonyms

Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor



经过测试的应用

Positive IF detected inhuman breast cancer tissue
Planning an IHC experiment? We recommend our IHCeasy TNF Ready-To-Use IHC Kit. TNF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 60291-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-60291 targets TNF Alpha in WB, IF applications and shows reactivity with human samples.

Tested Applications IF
Cited Applications IF, WB
Tested Reactivity human
Cited Reactivitymouse
Immunogen TNF Alpha fusion protein Ag11413 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name tumor necrosis factor (TNF superfamily, member 2)
Synonyms Cachectin, DIF, TNF, TNF a, TNF alpha, TNFA, TNFSF2, Tumor necrosis factor
Calculated Molecular Weight 233 aa, 26 kDa
GenBank Accession NumberBC028148
Gene Symbol TNF
Gene ID (NCBI) 7124
ENSEMBL Gene IDENSG00000232810
RRIDAB_2883136
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP01375
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, INS resistance, and cancer. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 TNF Alpha antibody CL488-60291Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

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WB

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Authors - Qing Xiong
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Authors - Qiumeng Chen