- Featured Product
- KD/KO Validated
CD71 Polyclonal antibody
CD71 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
IF/ICC, IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HT-1080 cells, HeLa cells, Jurkat cells, K-562 cells, human placenta tissue |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10084-2-AP targets CD71 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB,IHC,IF,Cell treatment |
Tested Reactivity | human |
Cited Reactivity | human, chicken, monkey, pig, duck |
Immunogen | CD71 fusion protein Ag0123 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | transferrin receptor (p90, CD71) |
Synonyms | CD71, p90, sTfR, T9, TFR, TFR1, TFRC, TR, Transferrin receptor protein 1, TRFR |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 90 kDa |
GenBank Accession Number | BC001188 |
Gene Symbol | TFRC |
Gene ID (NCBI) | 7037 |
ENSEMBL Gene ID | ENSG00000072274 |
RRID | AB_2240403 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02786 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CD71 antibody 10084-2-AP | Download protocol |
IHC protocol for CD71 antibody 10084-2-AP | Download protocol |
IF protocol for CD71 antibody 10084-2-AP | Download protocol |
IP protocol for CD71 antibody 10084-2-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. | ||
Acta Neuropathol Seizure-mediated iron accumulation and dysregulated iron metabolism after status epilepticus and in temporal lobe epilepsy. | ||
Cell Death Differ Ferroptotic stress facilitates smooth muscle cell dedifferentiation in arterial remodelling by disrupting mitochondrial homeostasis | ||
Acta Biomater Cyclosporine A-loaded apoferritin alleviates myocardial ischemia-reperfusion injury by simultaneously blocking ferroptosis and apoptosis of cardiomyocytes | ||
Cancer Res Intratumoral payload release influences the potency and bystander-killing effects of antibody-drug conjugates in preclinical models. | ||