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RCC1 Polyclonal antibody

RCC1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 10697-1-AP

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Synonyms

RCC1, SNHG3 RCC1, SNHG3-RCC1



经过测试的应用

Positive WB detected inA431 cells, Jurkat cells, mouse testis tissue, Raji cells, SGC-7901 cells, HeLa cells, HEK-293 cells, HEK-293T cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human urothelial carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10697-1-AP targets RCC1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen RCC1 fusion protein Ag1042 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SNHG3-RCC1 readthrough transcript
Synonyms RCC1, SNHG3 RCC1, SNHG3-RCC1
Calculated Molecular Weight 45 kDa, 48 kDa
Observed Molecular Weight 68 kDa
GenBank Accession NumberBC007300
Gene Symbol SNHG3-RCC1
Gene ID (NCBI) 751867
RRIDAB_513962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP18754
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

实验方案

Product Specific Protocols
WB protocol for RCC1 antibody 10697-1-APDownload protocol
IHC protocol for RCC1 antibody 10697-1-APDownload protocol
IP protocol for RCC1 antibody 10697-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols