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- KD/KO Validated
SIX2 Polyclonal antibody
SIX2 Polyclonal Antibody for IF, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (6)
Applications
IF, IP, ELISA and More (2)
Conjugate
Unconjugated
245
验证数据展示
经过测试的应用
Positive IP detected in | HEK-293 cells |
Positive IF detected in | mouse embryonic kidney, heritable frontonasal dysplasia and renal hypoplasia in 3H1 Br mice |
推荐稀释比
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11562-1-AP targets SIX2 in WB, IP, IF, ChIP, ELISA applications and shows reactivity with human samples.
Tested Applications | IF, IP, ELISA |
Cited Applications | ChIP, IP, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, rabbit, pig, Alligator, canine |
Immunogen | SIX2 fusion protein Ag2124 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SIX homeobox 2 |
Synonyms | Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2 |
Calculated Molecular Weight | 291 aa, 32 kDa |
Observed Molecular Weight | 32-37 kDa |
GenBank Accession Number | BC024033 |
Gene Symbol | SIX2 |
Gene ID (NCBI) | 10736 |
RRID | AB_2189084 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9NPC8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SIX2 antibody 11562-1-AP | Download protocol |
IP protocol for SIX2 antibody 11562-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature Kidney organoids from human iPS cells contain multiple lineages and model human nephrogenesis. | ||
Nat Biotechnol Tubuloids derived from human adult kidney and urine for personalized disease modeling. | ||
Nat Biotechnol Revealing the spatio-phenotypic patterning of cells in healthy and tumor tissues with mLSR-3D and STAPL-3D. | ||
Cell In Vivo Target Gene Activation via CRISPR/Cas9-Mediated Trans-epigenetic Modulation. | ||
Cell Stem Cell Redefining the in vivo origin of metanephric nephron progenitors enables generation of complex kidney structures from pluripotent stem cells. |