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- KD/KO Validated
CD138/Syndecan-1 Polyclonal antibody
CD138/Syndecan-1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
IHC, WB, ELISA and More (3)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse spleen tissue, HeLa cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:800-1:3200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10593-1-AP targets CD138/Syndecan-1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, CoIP |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, pig |
| Immunogen | CD138/Syndecan-1 fusion protein Ag0823 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | syndecan 1 |
| Synonyms | CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1 |
| Calculated Molecular Weight | 32.5 kDa |
| Observed Molecular Weight | 60-100 kDa, 130 kDa |
| GenBank Accession Number | BC008765 |
| Gene Symbol | SDC1 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_2182736 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P18827 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CD138/Syndecan-1 antibody 10593-1-AP | Download protocol |
| IHC protocol for CD138/Syndecan-1 antibody 10593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Autophagy SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes | ||
Theranostics Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production. | ||
Mol Neurodegener Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model. | ||
Cell Mol Life Sci The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium. |
