SAE1 Polyclonal antibody

SAE1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 10229-1-AP

Print datasheet

Synonyms

AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A



经过测试的应用

Positive WB detected inA431 cells, A549 cells, COLO 320 cells, HEK-293 cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10229-1-AP targets SAE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SAE1 fusion protein Ag0275 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SUMO1 activating enzyme subunit 1
Synonyms AOS1, FLJ3091, HSPC140, SAE1, SUA1, UBLE1A
Calculated Molecular Weight 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC000344
Gene Symbol SAE1
Gene ID (NCBI) 10055
RRIDAB_2182917
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBE0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. It had an apparent molecular mass of 40 kD by SDS-PAGE. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).

实验方案

Product Specific Protocols
WB protocol for SAE1 antibody 10229-1-APDownload protocol
IHC protocol for SAE1 antibody 10229-1-APDownload protocol
IF protocol for SAE1 antibody 10229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Curr Biol

Pumilio 1 suppresses multiple activators of p53 to safeguard spermatogenesis.

Authors - Dong Chen
humanIHC

Open Med (Wars)

Bioinformatics-based analysis of SUMOylation-related genes in hepatocellular carcinoma reveals a role of upregulated SAE1 in promoting cell proliferation.

Authors - Yang Liu
humanWB

Nat Commun

SENP1 prevents steatohepatitis by suppressing RIPK1-driven apoptosis and inflammation

Authors - Lingjie Yan
humanIHC,WB

Curr Issues Mol Biol

SUMO-Activating Enzyme Subunit 1 Is Associated with Poor Prognosis, Tumor Progression, and Radio-Resistance in Colorectal Cancer

Authors - Yueh-Jung Wu
humanWB

Elife

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

Authors - Xiancai Ma