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  • KD/KO Validated

Rab23 Polyclonal antibody

Rab23 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 11101-1-AP

Print datasheet

Synonyms

HSPC137, RAB23, Ras related protein Rab 23



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue, HEK-293 cells, human brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11101-1-AP targets Rab23 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Rab23 fusion protein Ag1588 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name RAB23, member RAS oncogene family
Synonyms HSPC137, RAB23, Ras related protein Rab 23
Calculated Molecular Weight 237 aa, 27 kDa
Observed Molecular Weight 27 kDa
GenBank Accession NumberBC015021
Gene Symbol RAB23
Gene ID (NCBI) 51715
RRIDAB_2173784
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULC3
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Rab23 is a novel member of large Rab small GTPase family. It plays important roles during embryonic development. The brain-enriched Rab23 is the only Rab protein that is known to have a distinct function during central nervous system development, playing an essential role as a negative regulator of the Sonic Hedgehog (Shh) pathway. RAB23 has recently been identified in mesangial cells (MCs), and could serve as a biomarker that indicates the severity of FSGS ( focal segmental glomerulosclerosis). Mutations in RAB23 may result in ACPS2 ( acrocephalopolysyndactyly type 2).

实验方案

Product Specific Protocols
WB protocol for Rab23 antibody 11101-1-APDownload protocol
IHC protocol for Rab23 antibody 11101-1-APDownload protocol
IP protocol for Rab23 antibody 11101-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Curr Biol

Planar Cell Polarity Effector Proteins Inturned and Fuzzy Form a Rab23 GEF Complex.

Authors - Andreas Gerondopoulos
  • KD Validated
mouseWB,IHC

Elife

RAB23 coordinates early osteogenesis by repressing FGF10-pERK1/2 and GLI1.

Authors - Md Rakibul Hasan
humanWB,IF

Oncotarget

Rab23 promotes squamous cell carcinoma cell migration and invasion via integrin β1/Rac1 pathway.

Authors - Qiang Jian
  • KD Validated
humanWB

J Cell Sci

Differential role of Rab proteins in ciliary trafficking: Rab23 regulates smoothened levels.

Authors - Boehlke Christopher C
  • KD Validated
humanWB,IHC

J Cancer Res Clin Oncol

MicroRNA-367-3p directly targets RAB23 and inhibits proliferation, migration and invasion of bladder cancer cells and increases cisplatin sensitivity

Authors - Xifeng Wei
  • KD Validated
human,mouseWB,IF

Exp Dermatol

Silencing of Rab23 by siRNA inhibits ultraviolet B-induced melanogenesis via downregulation of PKA/CREB/MITF.

Authors - Xuanjin Wei
  • KD Validated