• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Poliovirus receptor Polyclonal antibody

Poliovirus receptor Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27486-1-AP

Print datasheet

Synonyms

CD155, HVED, Necl 5, NECL5, Nectin like protein 5, Poliovirus receptor, PVR, PVS, TAGE4

验证数据展示

  • WB analysis of HT-1080 using 27486-1-AP

    WB analysis of HT-1080 using 27486-1-AP

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 27486-1-AP (PVR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 27486-1-AP

    IHC staining of human cervical cancer using 27486-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PVR antibody included
  • IHC staining of human cervical cancer using 27486-1-AP

    IHC staining of human cervical cancer using 27486-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 27486-1-AP (Poliovirus receptor antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with PVR antibody included

经过测试的应用

Positive WB detected inHT-1080 cells, HUVEC cells, A549 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy PVR Ready-To-Use IHC Kit. PVR primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27486-1-AP targets Poliovirus receptor in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen Poliovirus receptor fusion protein Ag26657 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name poliovirus receptor
Synonyms CD155, HVED, Necl 5, NECL5, Nectin like protein 5, Poliovirus receptor, PVR, PVS, TAGE4
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC015542
Gene Symbol PVR
Gene ID (NCBI) 5817
RRIDAB_2880884
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP15151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Poliovirus receptor antibody 27486-1-APDownload protocol
IHC protocol for Poliovirus receptor antibody 27486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
View Article