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  • KD/KO Validated

PTPIP51 Polyclonal antibody

PTPIP51 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 20641-1-AP

Print datasheet

Synonyms

FAM82A2, FAM82C, hRMD 3, Protein FAM82A2, Protein FAM82C, PTPIP51, RMD 3, RMD3



经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman skin tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20641-1-AP is PTPIP51 Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications COIP, IF, IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, rat, monkey
Immunogen PTPIP51 fusion protein Ag14708 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name family with sequence similarity 82, member A2
Synonyms FAM82A2, FAM82C, hRMD 3, Protein FAM82A2, Protein FAM82C, PTPIP51, RMD 3, RMD3
Calculated Molecular Weight 470 aa, 52 kDa
Observed Molecular Weight 52-60 kDa
GenBank Accession NumberBC008970
Gene Symbol FAM82A2
Gene ID (NCBI) 55177
RRIDAB_10695187
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96TC7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PTPIP51 antibody 20641-1-APDownload protocol
IHC protocol for PTPIP51 antibody 20641-1-APDownload protocol
IF protocol for PTPIP51 antibody 20641-1-APDownload protocol
IP protocol for PTPIP51 antibody 20641-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Metab

A High-Density Human Mitochondrial Proximity Interaction Network.

Authors - Hana Antonicka
monkeyWB,IF

Nat Cell Biol

MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.

Authors - Tim König
  • KD Validated
humanCOIP

Mol Cell

The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.

Authors - Yan G Zhao
humanWB

J Cell Biol

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Authors - Nan Liu
humanWB

J Cell Sci

The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.

Authors - Song Han
mouseIF

Sci Rep

Proteomics-Based Approach Identifies Altered ER Domain Properties by ALS-Linked VAPB Mutation.

Authors - Tomoyuki Yamanaka