AMPK Gamma 2 Polyclonal antibody

AMPK Gamma 2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, WB, ELISA

Conjugate

Unconjugated

Cat No : 12568-1-AP

Print datasheet

Synonyms

AAKG, AAKG2, AMPK Gamma 2, AMPK gamma2, AMPK subunit gamma 2, AMPKγ2, CMH6, H91620p, PRKAG2, WPWS



经过测试的应用

Positive WB detected inHEK-293 cells, PC-3 cells, mouse eye tissue, HeLa cells, Neuro-2a cells, mouse heart tissues, rat heart tissues
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12568-1-AP targets AMPK Gamma 2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AMPK Gamma 2 fusion protein Ag3214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name protein kinase, AMP-activated, gamma 2 non-catalytic subunit
Synonyms AAKG, AAKG2, AMPK Gamma 2, AMPK gamma2, AMPK subunit gamma 2, AMPKγ2, CMH6, H91620p, PRKAG2, WPWS
Calculated Molecular Weight 569 aa, 63 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC020540
Gene Symbol PRKAG2
Gene ID (NCBI) 51422
RRIDAB_2252960
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

实验方案

Product Specific Protocols
WB protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
IF protocol for AMPK Gamma 2 antibody 12568-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Med Chem

Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.

Authors - Yuwen Sheng
humanWB,IF

J Cardiol

Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.

Authors - Zhang Bi-Li BL