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PI3 Kinase p55 Gamma Polyclonal antibody

PI3 Kinase p55 Gamma Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27035-1-AP

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Synonyms

p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3



经过测试的应用

Positive WB detected inMCF-7 cells, HUVEC cells, HEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse testis tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27035-1-AP targets PI3 Kinase p55 Gamma in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen PI3 Kinase p55 Gamma fusion protein Ag25884 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoinositide-3-kinase, regulatory subunit 3 (gamma)
Synonyms p55, p55 GAMMA, p55PIK, PI3 Kinase p55 Gamma, PI3 kinase subunit p55 gamma, PI3K regulatory subunit gamma, PIK3R3
Calculated Molecular Weight 85 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC021622
Gene Symbol PIK3R3
Gene ID (NCBI) 8503
RRIDAB_2880730
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PIK3R3, also named as p55PIK, belongs to the PI3K p85 subunit family. It binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During INS stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The antibody is specific to PIK3R3.

实验方案

Product Specific Protocols
WB protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
IHC protocol for PI3 Kinase p55 Gamma antibody 27035-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Am J Physiol Lung Cell Mol Physiol

p55PIK Deficiency and its N-terminal Derivative inhibit Inflammation and Emphysema in COPD mouse model.

Authors - Ting Wang
human,mouseWB,IHC

BMC Cancer

Circ_0021350 plays an oncogene role by regulating miR-1207-3p/PIK3R3 in glioblastoma

Authors - Cheng Tan
  • KD Validated