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PF4 Polyclonal antibody

PF4 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21157-1-AP

Print datasheet

Synonyms

C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4

验证数据展示

  • WB analysis of human peripheral blood platelets using 21157-1-AP

    WB analysis of human peripheral blood platelets using 21157-1-AP

    Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 21157-1-AP (PF4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse spleen using 21157-1-AP

    IHC staining of mouse spleen using 21157-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 21157-1-AP (PF4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 21157-1-AP

    IF Staining of HeLa using 21157-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PF4 antibody (21157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHuman peripheral blood platelets tissue, mouse spleen tissue
Positive IHC detected inmouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21157-1-AP targets PF4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity human, mouse, pig
Cited Reactivityhuman, mouse
Immunogen PF4 fusion protein Ag12345 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name platelet factor 4
Synonyms C X C motif chemokine 4, CXCL4, Iroplact, Oncostatin A, PF 4, PF4, platelet factor 4, SCYB4
Calculated Molecular Weight 101 aa, 11 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC093965
Gene Symbol PF4
Gene ID (NCBI) 5196
RRIDAB_2878819
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02776
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation

实验方案

Product Specific Protocols
WB protocol for PF4 antibody 21157-1-APDownload protocol
IHC protocol for PF4 antibody 21157-1-APDownload protocol
IF protocol for PF4 antibody 21157-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IHC

J Virol

Chemokine PF4 Inhibits EV71 and CA16 Infections at the Entry Stage.

Authors - Zhichao Pei
View Article
humanIF

Cell Biosci

Neutrophil extracellular traps promote thrombogenicity in cerebral venous sinus thrombosis.

Authors - Jiaqi Jin
View Article
humanWB

Amyloid

Binding of serum-derived amyloid-associated proteins to amyloid fibrils

Authors - Yohei Misumi
View Article
humanWB,IF

J Vasc Access

Functions for platelet factor 4 (PF4/CXCL4) and its receptors in fibroblast-myofibroblast transition and fibrotic failure of arteriovenous fistulas (AVFs)

Authors - Yuxuan Xiao
View Article