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  • Featured Product
  • KD/KO Validated

PEA15 Polyclonal antibody

PEA15 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 21446-1-AP

Print datasheet

Synonyms

HMAT1, HUMMAT1H, MAT1, MAT1H, PEA 15, PEA15, PED

验证数据展示

  • IHC staining of mouse brain using 21446-1-AP

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 21446-1-AP

    IHC staining of mouse brain using 21446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21446-1-AP (PEA15 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of fetal human brain using 21446-1-AP

    WB analysis of fetal human brain using 21446-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21446-1-AP (PEA15 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inmouse brain tissue, human heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21446-1-AP is PEA15 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen PEA15 fusion protein Ag13782 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphoprotein enriched in astrocytes 15
Synonyms HMAT1, HUMMAT1H, MAT1, MAT1H, PEA 15, PEA15, PED
Calculated Molecular Weight 130 aa, 15 kDa
Observed Molecular Weight 15-18 kDa
GenBank Accession NumberBC002426
Gene Symbol PEA15
Gene ID (NCBI) 8682
RRIDAB_2878858
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15121
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PEA15 antibody 21446-1-APDownload protocol
IHC protocol for PEA15 antibody 21446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cancer

The microRNA212 regulated PEA15 promotes ovarian cancer progression by inhibiting of apoptosis.

Authors - Yonghong Luo
  • KD Validated
View Article