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PD-1/CD279 Antibody 4 Publications

Rabbit Polyclonal| Catalog number: 18106-1-AP

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浓度: 250μg/ml

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Related Primary Antibodies

Tested Applications

Applications:
WB, IHC, IF, ELISA
Application not tested? Please see our Guarantee


Images See all

PD-1/CD279 Antibody WB mouse thymus tissue 18106-1-AP

mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

PD-1/CD279 Antibody IHC human tonsillitis tissue 18106-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PD-1/CD279 Antibody IHC human tonsillitis tissue 18106-1-AP

Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

PD-1/CD279 Antibody IF human tonsillitis tissue 18106-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed human tonsillitis tissue using 18106-1-AP(PD-1/CD279 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

PD-1/CD279 Antibody IF human tonsillitis tissue 18106-1-AP

Immunofluorescent analysis of ( 4% PFA ) fixed human tonsillitis tissue using 18106-1-AP(PD-1/CD279 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, mouse
Positive WB detected in:
mouse thymus tissue, mouse spleen tissue
Positive IHC detected in:
human tonsillitis tissue, human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human tonsillitis tissue
Recommended dilution:
WB : 1:300-1:1000
IHC : 1:400-1:1600
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
PD-1/CD279 fusion protein Ag12470
Full name:
programmed cell death 1
Calculated molecular weight:
288aa,32 kDa
Observed molecular weight:
47-55 kDa
GenBank accession number:
BC074740
Gene ID (NCBI):
5133
Gene symbol
PDCD1
Synonyms
CD279, hPD 1, hPD l, PD 1, PD1, PD-1, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

Background

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).


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