- Featured Product
- KD/KO Validated
OPA1 Polyclonal antibody
OPA1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, rat, mouse and More (8)
Applications
IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
109
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse brain tissue, HEK-293 cells, rat brain tissue, HEK-293T cells, HeLa cells, mouse liver tissue, HepG2 cells |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse heart tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
27733-1-AP targets OPA1 in WB,IP,IHC,ELISA applications and shows reactivity with Human, rat, mouse samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB,IHC,IF,CoIP |
Tested Reactivity | Human, rat, mouse |
Cited Reactivity | human, mouse, rat, goat, Chicken, zebrafish, hamster, pig, duck, bovine |
Immunogen | OPA1 fusion protein Ag26887 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | optic atrophy 1 (autosomal dominant) |
Synonyms | KIAA0567, largeG, MGM1, NTG, OPA1, Optic atrophy protein 1 |
Calculated Molecular Weight | 960 aa, 112 kDa |
Observed Molecular Weight | 100 kDa, 80-90 kDa |
GenBank Accession Number | BC075805 |
Gene Symbol | OPA1 |
Gene ID (NCBI) | 4976 |
RRID | AB_2810292 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60313 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for OPA1 antibody 27733-1-AP | Download protocol |
IHC protocol for OPA1 antibody 27733-1-AP | Download protocol |
IP protocol for OPA1 antibody 27733-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
ACS Cent Sci Macrophage Inactivation by Small Molecule Wedelolactone via Targeting sEH for the Treatment of LPS-Induced Acute Lung Injury | ||
Mol Cell Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation | ||
Mol Cell Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.
| ||
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers | ||
Nat Commun PINCH-1 regulates mitochondrial dynamics to promote proline synthesis and tumor growth. |