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  • KD/KO Validated

NXF1 Polyclonal antibody

NXF1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IF, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10328-1-AP

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Synonyms

MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein



经过测试的应用

Positive WB detected inHeLa cells, HEK-293T cells, K-562 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10328-1-AP targets NXF1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications IF, IP, WB, ELISA
Cited ApplicationsCoIP, IF, IP, WB
Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Immunogen NXF1 fusion protein Ag0413 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear RNA export factor 1
Synonyms MEX67, mRNA export factor TAP, nuclear RNA export factor 1, NXF1, TAP, Tip associated protein, Tip associating protein
Calculated Molecular Weight 70 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC004904
Gene Symbol NXF1
Gene ID (NCBI) 10482
RRIDAB_2236500
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBU9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

实验方案

Product Specific Protocols
WB protocol for NXF1 antibody 10328-1-APDownload protocol
IF protocol for NXF1 antibody 10328-1-APDownload protocol
IP protocol for NXF1 antibody 10328-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Biol

Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.

Authors - Zhang Liang L
  • KD Validated

J Cell Biol

Intronless mRNAs transit through nuclear speckles to gain export competence.

Authors - Ke Wang
humanWB

Nucleic Acids Res

Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.

Authors - Sarah H Stubbs
humanWB

PLoS Genet

Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.

Authors - Miao Chen
  • KD Validated
monkeyWB,CoIP

Mol Cell Biol

Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.

Authors - Kim Miri M
  • KD Validated
humanWB,IF,IP

Chromosoma

XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.

Authors - Cohen Hannah R HR