NRF1/nuclear respiratory factor 1 Monoclonal antibody

NRF1/nuclear respiratory factor 1 Monoclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

FC, IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C2B8

Cat No : 66832-1-Ig

Print datasheet

Synonyms

ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy NRF1 Ready-To-Use IHC Kit. NRF1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

66832-1-Ig targets NRF1/nuclear respiratory factor 1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IF, IHC, WB, ELISA
Cited Applications WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NRF1/nuclear respiratory factor 1 fusion protein Ag28149 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name nuclear respiratory factor 1
Synonyms ALPHA PAL, NRF 1, NRF1, nuclear respiratory factor 1
Calculated Molecular Weight 522 aa, 56 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC016925
Gene Symbol NRF1
Gene ID (NCBI) 4899
RRIDAB_2882175
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16656
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NRF1, also named as Alpha-pal and NRF-1, belongs to the NRF1/Ewg family. It is a transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. NRF1 is implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. It plays a critical role in regulating the expression of many antioxidant response element (ARE)-dependent genes. NRF1 exists various isoforms and long isoform is about 67 kDa and short isoform is about 45 kDa.

实验方案

Product Specific Protocols
WB protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
IHC protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
IF protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
FC protocol for NRF1/nuclear respiratory factor 1 antibody 66832-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Clin Transl Hepatol

Icaritin Attenuates Lipid Accumulation by Increasing Energy Expenditure and Autophagy Regulated by Phosphorylating AMPK.

Authors - Yue Wu
ratWB

PLoS One

Supplementation with dietary omega-3 PUFA mitigates fetal brain inflammation and mitochondrial damage caused by high doses of sodium nitrite in maternal rats.

Authors - Jingchi Sun
mouseWB

Nitric Oxide

Hydrogen sulfide alleviates heart failure with preserved ejection fraction in mice by targeting mitochondrial abnormalities via PGC-1α

Authors - Shuying Huang
ratWB

J Pharm Pharmacol

Styrax (Liquidambar orientalis Mill.) promotes mitochondrial function and reduces cardiac damage following myocardial ischemic injury: the role of the AMPK-PGC1α signaling pathway

Authors - Fei Mu
humanWB

Folia Biol (Praha)

NRF1 Alleviated Oxidative Stress of Glioblastoma Cells by Regulating NOR1

Authors - Jiali Wang