• Featured Product
  • KD/KO Validated

B23/NPM1 Polyclonal antibody

B23/NPM1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (2)

Applications

IF, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 10306-1-AP

Print datasheet

Synonyms

B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin



经过测试的应用

Positive WB detected inCOLO 320 cells, Jurkat cells, multi-cells, K-562 cells, HeLa cells, HEK-293 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NPM1 Ready-To-Use IHC Kit. NPM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10306-1-AP targets B23/NPM1 in WB, IP, IHC, IF, CoIP, chIP, ELISA applications and shows reactivity with human, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications chIP, CoIP, IF, IHC, IP, WB
Tested Reactivity human, rat
Cited Reactivityhuman, mouse, pig
Immunogen B23/NPM1 fusion protein Ag0286 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nucleophosmin (nucleolar phosphoprotein B23, numatrin)
Synonyms B23, B23/NPM1, NPM1, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Nucleophosmin, Numatrin
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC002398
Gene Symbol NPM1
Gene ID (NCBI) 4869
RRIDAB_2155163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP06748
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nucleophosmin (NPM1,B23) is a putative ribosome assembly factor with a high affinity for peptides containing nuclear localization signals (NLSs). The transport of proteins across the nuclear envelope is a selective, multistep process involving several cytoplasmic factors. Proteins must be recognized as import substrates, dock at the nuclear pore complex and translocate across the nuclear envelope in an ATP-dependent fashion. Several cytosolic and nuclear proteins that are central to this process have been identified. The 38 kDa nuclear protein nucleophosmin is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis.

实验方案

Product Specific Protocols
WB protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IHC protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IF protocol for B23/NPM1 antibody 10306-1-APDownload protocol
IP protocol for B23/NPM1 antibody 10306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
mouse

EMBO Mol Med

Excess hydrogen sulfide and polysulfides production underlies a schizophrenia pathophysiology.

Authors - Masayuki Ide
humanchIP

Cancer Res

LNMICC promotes nodal metastasis of cervical cancer by reprogramming fatty acid metabolism.

Authors - Chunliang Shang
humanIF

Int J Mol Sci

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Authors - Ahmed Hassan
humanWB,IF

Mol Ther Nucleic Acids

RPL32 Promotes Lung Cancer Progression by Facilitating p53 Degradation.

Authors - Jiansheng Xie
humanWB

iScience

Elevated high-mannose N-glycans hamper endometrial decidualization

Authors - Siyi Chen
  • KD Validated