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NFKB2 Polyclonal antibody

NFKB2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 51085-1-AP

Print datasheet

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52

验证数据展示

  • WB analysis of Apoptosised HeLa using 51085-1-AP

    WB analysis of Apoptosised HeLa using 51085-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 51085-1-AP (NFKB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inApoptosised HeLa cells
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

51085-1-AP targets NFKB2 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 103 kDa
GenBank Accession NumberBC002844
Gene Symbol NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
RRIDAB_2267124
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NFKB2.

实验方案

Product Specific Protocols
WB protocol for NFKB2 antibody 51085-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols