NFKB2,p52,p100-Specific Polyclonal antibody

NFKB2,p52,p100-Specific Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15503-1-AP

Print datasheet

Synonyms

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52



经过测试的应用

Positive WB detected inA431 cells, HeLa cells, MCF-7 cells, mouse heart tissue
Positive IHC detected inhuman testis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy NFKB2 Ready-To-Use IHC Kit. NFKB2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15503-1-AP targets NFKB2,p52,p100-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
Synonyms DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, NFKB2,p52,p100, Oncogene Lyt 10, p100, p52
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 52/100 kDa
GenBank Accession NumberNM_001077494
Gene Symbol NFKB2
Gene ID (NCBI) 4791
ENSEMBL Gene IDENSG00000077150
RRIDAB_2267126
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ00653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind both p52 and p100 isoforms of NFKB2.

实验方案

Product Specific Protocols
WB protocol for NFKB2,p52,p100-Specific antibody 15503-1-APDownload protocol
IHC protocol for NFKB2,p52,p100-Specific antibody 15503-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cancers (Basel)

Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.

Authors - Ha-Yeon Shin
humanIF,WB

Sci Rep

TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway.

Authors - Jun Wang
humanWB

Pharm Biol

Simiao Qingwen Baidu decoction inhibits Epstein-Barr virus-induced B lymphoproliferative disease and lytic viral replication.

Authors - Xianhui Yang
humanWB

Mol Cell Endocrinol

Interleukin-1 increases cyclooxygenase-2 expression and prostaglandin E2 production in human granulosa-lutein cell via nuclear factor kappa B/P65 and extracellular signal-regulated kinase 1/2 signaling pathways

Authors - Shan Wan
  • KD Validated
humanWB

Theranostics

Platelet membrane-coated alterbrassicene A nanoparticle inhibits calcification of the aortic valve by suppressing phosphorylation P65 NF-κB

Authors - Bingchuan Geng