NDNL2 Polyclonal antibody

NDNL2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IF, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 27488-1-AP

Print datasheet

Synonyms

HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3



经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, HeLa cells, human placenta tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27488-1-AP targets NDNL2 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IF, IHC, WB,ELISA
Cited Applications WB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen NDNL2 fusion protein Ag26663 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name necdin-like 2
Synonyms HCA4, MAGE G1 antigen, MAGEG1, MAGEL3, Melanoma associated antigen G1, NDNL2, necdin like 2, Necdin like protein 2, NSE3, NSMCE3
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC053999
Gene Symbol NDNL2
Gene ID (NCBI) 56160
RRIDAB_2880885
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96MG7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDNL2 (also known as NSMCE3 or MAGEG1) is a part of the SMC5-6 protein complex that is essential for DNA damage response and chromosome segregation (PMID: 18086888; 27427983). The gene of human NDNL2 maps to chromosome 15q13.1. Northern blot analysis detected expression of a 1.9-kb transcript in all human tissues tested, with highest expression in testis (PMID: 11782285). Missense mutations in NSMCE3 have been associated with an autosomal recessive chromosome breakage syndrome that leads to defective T and B cell function and acute respiratory distress syndrome in early childhood (PMID: 27427983).

实验方案

Product Specific Protocols
WB protocol for NDNL2 antibody 27488-1-APDownload protocol
IHC protocol for NDNL2 antibody 27488-1-APDownload protocol
IF protocol for NDNL2 antibody 27488-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

Epstein-Barr virus BNRF1 destabilizes SMC5/6 cohesin complexes to evade its restriction of replication compartments.

Authors - Stephanie Pei Tung Yiu