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  • KD/KO Validated

NDE1 Polyclonal antibody

NDE1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10233-1-AP

Print datasheet

Synonyms

HOM TES 87, NDE1, NUDE, NUDE1



经过测试的应用

Positive WB detected inA2780 cells, HEK-293 cells, HeLa cells, rat liver tissue, mouse liver tissue, HepG2 cells, mouse brain tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10233-1-AP targets NDE1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen NDE1 fusion protein Ag0336 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nudE nuclear distribution gene E homolog 1 (A. nidulans)
Synonyms HOM TES 87, NDE1, NUDE, NUDE1
Calculated Molecular Weight 335 aa, 38 kDa
Observed Molecular Weight 40 kDa
GenBank Accession NumberBC001421
Gene Symbol NDE1
Gene ID (NCBI) 54820
RRIDAB_2149877
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXR1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDE1 (NUDE /NUDE1) is a LIS1-interacting protein. A signaling pathway containing NDE1, LIS1, and cytoplasmic dynein is conserved for eukaryotic nuclear migration and has profound impact on neuronal migration during development of the central nervous system. LIS1 and dynein have been shown to be involved in mitosis and NDE1 participates in a subset of dynein function in M phase. NDE1 is specifically phosphorylated in M phase in its serine/threonine phosphorylation motifs, probably by Cdc2 and also Erk1 and Erk2. A fraction of NDEl binds to centrosomes strongly in interphase and is localized to mitotic spindles in early M phase. Phosphorylation of NDE1 also regulates the cell-cycle-dependent distribution, possibly by increasing its dissociation rate at the microtubule-organizing center. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human NDE1.

实验方案

Product Specific Protocols
WB protocol for NDE1 antibody 10233-1-APDownload protocol
IHC protocol for NDE1 antibody 10233-1-APDownload protocol
IF protocol for NDE1 antibody 10233-1-APDownload protocol
IP protocol for NDE1 antibody 10233-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
human,mouseWB,IF

Nat Cell Biol

Nde1-mediated inhibition of ciliogenesis affects cell cycle re-entry.

Authors - Kim Sehyun S
  • KD Validated
humanWB

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
human,mouseWB,IHC,IF

Am J Hum Genet

The essential role of centrosomal NDE1 in human cerebral cortex neurogenesis.

Authors - Bakircioglu Mehmet M
humanWB

J Cell Biol

Mitotic control of kinetochore-associated dynein and spindle orientation by human Spindly.

Authors - Chan Ying Wai YW
humanWB,IF

EMBO J

CPAP promotes timely cilium disassembly to maintain neural progenitor pool.

Authors - Elke Gabriel