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NBN / NBS1 Polyclonal antibody
NBN / NBS1 Polyclonal Antibody for IF/ICC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, human testis tissue |
Positive IP detected in | HeLa cells |
Positive IF detected in | A549 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2400 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
55025-1-AP targets NBN / NBS1 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, CoIP |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | nibrin |
Synonyms | AT V1, AT V2, ATV, NBN, NBS, NBS1, nibrin, P95 |
Calculated Molecular Weight | 85 kDa |
Observed Molecular Weight | 90-95 kDa |
GenBank Accession Number | NM_002485 |
Gene Symbol | NBN |
Gene ID (NCBI) | 4683 |
RRID | AB_10858928 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O60934 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for NBN / NBS1 antibody 55025-1-AP | Download protocol |
IF protocol for NBN / NBS1 antibody 55025-1-AP | Download protocol |
IP protocol for NBN / NBS1 antibody 55025-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mol Cell C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex. | ||
Nat Commun Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin | ||
Nucleic Acids Res LncRNA lnc-RI regulates homologous recombination repair of DNA double-strand breaks by stabilizing RAD51 mRNA as a competitive endogenous RNA. | ||
Cell Death Differ NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis.
| ||
Oncogene TRIM24 is critical for the cellular response to DNA double-strand breaks through regulating the recruitment of MRN complex | ||