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MYH14 Monoclonal antibody

MYH14 Monoclonal Antibody for FC, IF/ICC, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse and More (1)

Applications

FC, IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

CloneNo.

1B4C9

Cat No : 66825-1-Ig

Print datasheet

Synonyms

DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C

验证数据展示

  • WB analysis of COLO 320 using 66825-1-Ig

    WB analysis of COLO 320 using 66825-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66825-1-Ig (MYH14 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 66825-1-Ig

    IHC staining of human colon using 66825-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH14 antibody included
  • IHC staining of human colon using 66825-1-Ig

    IHC staining of human colon using 66825-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66825-1-Ig (MYH14 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MYH14 antibody included
  • IF Staining of mouse skeletal muscle using 66825-1-Ig

    IF Staining of mouse skeletal muscle using 66825-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 66825-1-Ig (MYH14 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of HT-29 using 66825-1-Ig

    FC experiment of HT-29 using 66825-1-Ig

    1X10^6 HT-29 cells were intracellularly stained with 0.2 ug Anti-Human MYH14 (66825-1-Ig, Clone:1B4C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

经过测试的应用

Positive WB detected inCOLO 320 cells, HT-29 cells
Positive IHC detected inhuman colon tissue, mouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse skeletal muscle tissue
Positive FC detected inHT-29 cells
Planning an IHC experiment? We recommend our IHCeasy MYH14 Ready-To-Use IHC Kit. MYH14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:100000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66825-1-Ig targets MYH14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB,ELISA
Cited Applications IF, WB
Tested Reactivity Human, mouse
Cited Reactivityrat
Immunogen MYH14 fusion protein Ag28063 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 14
Synonyms DFNA4, KIAA2034, MHC16, MYH14, myosin, Myosin 14, Myosin heavy chain 14, myosin, heavy chain 14, NMHC II C
Calculated Molecular Weight 228 kDa
Observed Molecular Weight 200-230 kDa
GenBank Accession NumberNM_001077186
Gene Symbol MYH14
Gene ID (NCBI) 79784
RRIDAB_2882168
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ7Z406
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH14, also named as KIAA2034 and NMHC II-C, is cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Defects in MYH14 are the cause of deafness autosomal dominant type 4 (DFNA4).

实验方案

Product Specific Protocols
WB protocol for MYH14 antibody 66825-1-IgDownload protocol
IHC protocol for MYH14 antibody 66825-1-IgDownload protocol
IF protocol for MYH14 antibody 66825-1-IgDownload protocol
FC protocol for MYH14 antibody 66825-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

J Cell Sci

Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation

Authors - Helena Sofia Domingues
  • KD Validated
View Article
IF

J Cell Sci

Pushing myelination: developmental regulation of myosin expression drives oligodendrocyte morphological differentiation.

Authors - Helena Sofia Domingues
View Article