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METTL14 Polyclonal antibody

METTL14 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, ELISA and More (4)

Conjugate

Unconjugated

Cat No : 26158-1-AP

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Synonyms

KIAA1627, methyltransferase like 14, METTL14



经过测试的应用

Positive WB detected inA549 cells, HeLa cells, NCCIT cells, PC-3 cells
Planning an IHC experiment? We recommend our IHCeasy METTL14 Ready-To-Use IHC Kit. METTL14 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26158-1-AP targets METTL14 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Cited Applications IF, IHC, IP, RIP, WB
Tested Reactivity human
Cited Reactivityhuman, chicken, monkey
Immunogen METTL14 fusion protein Ag14325 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name methyltransferase like 14
Synonyms KIAA1627, methyltransferase like 14, METTL14
Calculated Molecular Weight 456 aa, 52 kDa
Observed Molecular Weight55-60 kDa
GenBank Accession NumberBC007449
Gene Symbol METTL14
Gene ID (NCBI) 57721
RRIDAB_2800447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9HCE5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

METTL14, is also named as Methyltransferase-like protein 14 or KIAA1627, is a 456 amino acid protein, which belongs to the MT-A70-like family and localized in the nucleus. The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues of some mRNAs and regulates the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability. M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naïve pluripotency-promoting transcripts results in transcript destabilization.

实验方案

Product Specific Protocols
WB protocol for METTL14 antibody 26158-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
WB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
humanWB

Mol Cell

mTORC1 stimulates cell growth through SAM synthesis and m6A mRNA-dependent control of protein synthesis.

Authors - Elodie Villa
WB

Neuron

Epitranscriptomic m6A Regulation of Axon Regeneration in the Adult Mammalian Nervous System.

Authors - Yi-Lan Weng
humanRIP

Nat Commun

The genomic landscape of cholangiocarcinoma reveals the disruption of post-transcriptional modifiers.

Authors - Yaodong Zhang