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MAGEC3 Polyclonal antibody

MAGEC3 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 21491-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 7.2, CT7.2, HCA2, MAGE C3, MAGE C3 antigen, MAGEC3, MAGEC4, melanoma antigen family C, 3, Melanoma associated antigen C3

验证数据展示

  • WB analysis of A375 using 21491-1-AP

    WB analysis of A375 using 21491-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 21491-1-AP (MAGEC3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of A375 using 21491-1-AP

    IF Staining of A375 using 21491-1-AP

    Immunofluorescent analysis of A375 cells using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 21491-1-AP

    IHC staining of human liver cancer using 21491-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 21491-1-AP

    IHC staining of human liver cancer using 21491-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 21491-1-AP (MAGEC3 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inA375 cells, mouse thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21491-1-AP is MAGEC3 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited Applications IP, WB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen MAGEC3 fusion protein Ag15819 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name melanoma antigen family C, 3
Synonyms Cancer/testis antigen 7.2, CT7.2, HCA2, MAGE C3, MAGE C3 antigen, MAGEC3, MAGEC4, melanoma antigen family C, 3, Melanoma associated antigen C3
Calculated Molecular Weight 643 aa, 72 kDa
Observed Molecular Weight 70-72 kDa
GenBank Accession NumberBC103893
Gene Symbol MAGEC3
Gene ID (NCBI) 139081
RRIDAB_10734430
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TD91
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MAGEC3 antibody 21491-1-APDownload protocol
IHC protocol for MAGEC3 antibody 21491-1-APDownload protocol
IF protocol for MAGEC3 antibody 21491-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
IP

Cancer Commun (Lond)

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

Authors - Qingnan Wu
View Article
humanWB

BMC Cancer

Post-transcriptional regulation of cancer/testis antigen MAGEC2 expression by TRIM28 in tumor cells.

Authors - Xiao Song
View Article