• Featured Product
  • KD/KO Validated

DYNC1H1 Polyclonal antibody

DYNC1H1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, zebrafish, mouse and More (1)

Applications

IF, IHC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 12345-1-AP

Print datasheet

Synonyms

DHC1, DHC1a, DNCH1, Dnchc1, DNCL, DNECL, DYHC, DYNC1H1, Dynein heavy chain, cytosolic, HL 3, KIAA0325, p22



经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, human brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12345-1-AP targets DYNC1H1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, rat, zebrafish, mouse samples.

Tested Applications IF, IHC, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, rat, zebrafish, mouse
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Immunogen DYNC1H1 fusion protein Ag2999 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dynein, cytoplasmic 1, heavy chain 1
Synonyms DHC1, DHC1a, DNCH1, Dnchc1, DNCL, DNECL, DYHC, DYNC1H1, Dynein heavy chain, cytosolic, HL 3, KIAA0325, p22
Calculated Molecular Weight 4646 aa, 532 kDa
Observed Molecular Weight 532 kDa
GenBank Accession NumberBC021297
Gene Symbol DYNC1H1
Gene ID (NCBI) 1778
RRIDAB_2261765
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14204
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. It can be divided into 2 large subgroups, namely, the axonemal and cytoplasmic dyneins. The conventional cytoplasmic dynein are comprised of 2 heavy chain polypeptides and a number of intermediate and light chains. DYNC1H1 is a cytoplasmic dynein and belongs to the dynein heavy chain family. It acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. DYNC1H1 has been implicated in the degeneration of dopaminergic neuron axons and motor neurons in PD patients..

实验方案

Product Specific Protocols
WB protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IHC protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IF protocol for DYNC1H1 antibody 12345-1-APDownload protocol
IP protocol for DYNC1H1 antibody 12345-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Science

Mechanism of spindle pole organization and instability in human oocytes.

Authors - Chun So
mouseIF

Science

A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.

Authors - Chun So
humanWB

Nature

Endophilin-A2 functions in membrane scission in clathrin-independent endocytosis.

Authors - Henri-François Renard
  • KD Validated
humanWB

Nat Cell Biol

Recycling of autophagosomal components from autolysosomes by the recycler complex.

Authors - Chuchu Zhou
human

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
humanWB

J Cell Biol

Ndel1 suppresses ciliogenesis in proliferating cells by regulating the trichoplein-Aurora A pathway.

Authors - Hironori Inaba
  • KD Validated