IRF3 Monoclonal antibody

IRF3 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (2)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1E6G8

Cat No : 66670-1-Ig

Print datasheet

Synonyms

interferon regulatory factor 3, IRF 3, IRF3



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, MOLT-4 cells, Jurkat cells, THP-1 cells, Daudi cells
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IRF3 Ready-To-Use IHC Kit. IRF3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66670-1-Ig targets IRF3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, WB
Tested Reactivity Human
Cited Reactivityhuman, mouse
Immunogen IRF3 fusion protein Ag27223 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name interferon regulatory factor 3
Synonyms interferon regulatory factor 3, IRF 3, IRF3
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC009395
Gene Symbol IRF3
Gene ID (NCBI) 3661
RRIDAB_2882024
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14653
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes.

实验方案

Product Specific Protocols
WB protocol for IRF3 antibody 66670-1-IgDownload protocol
IHC protocol for IRF3 antibody 66670-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Front Immunol

Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1.

Authors - Zhihai Zhou
humanWB

Vet Microbiol

DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation

Authors - Shujuan Xu
human,mouseWB

mBio

MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p

Authors - Kul Raj Rai
humanWB

Cell Commun Signal

Bacterial vesicles block viral replication in macrophages via TLR4-TRIF-axis

Authors - Jeff Bierwagen
humanWB

J Med Virol

SADS-CoV nsp1 inhibits the IFN-β production by preventing TBK1 phosphorylation and inducing CBP degradation

Authors - Yingjie Xiang
mouseWB

Autophagy

CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction

Authors - Hongyun Wang