IRF3 Monoclonal antibody
IRF3 Monoclonal Antibody for IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (2)
Applications
IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
1E6G8
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, MOLT-4 cells, Jurkat cells, THP-1 cells, Daudi cells |
Positive IHC detected in | human spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
IF | See 1 publications below |
产品信息
66670-1-Ig targets IRF3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | IHC, WB, ELISA |
Cited Applications | IF, WB |
Tested Reactivity | Human |
Cited Reactivity | human, mouse |
Immunogen | IRF3 fusion protein Ag27223 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | interferon regulatory factor 3 |
Synonyms | interferon regulatory factor 3, IRF 3, IRF3 |
Calculated Molecular Weight | 47 kDa |
Observed Molecular Weight | 50-60 kDa |
GenBank Accession Number | BC009395 |
Gene Symbol | IRF3 |
Gene ID (NCBI) | 3661 |
RRID | AB_2882024 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14653 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for IRF3 antibody 66670-1-Ig | Download protocol |
IHC protocol for IRF3 antibody 66670-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Front Immunol Swine Acute Diarrhea Syndrome Coronavirus Nucleocapsid Protein Antagonizes Interferon-β Production via Blocking the Interaction Between TRAF3 and TBK1. | ||
Vet Microbiol DDX56 antagonizes IFN-β production to enhance EMCV replication by inhibiting IRF3 nuclear translocation | ||
mBio MIR155HG Plays a Bivalent Role in Regulating Innate Antiviral Immunity by Encoding Long Noncoding RNA-155 and microRNA-155-5p | ||
Cell Commun Signal Bacterial vesicles block viral replication in macrophages via TLR4-TRIF-axis | ||
J Med Virol SADS-CoV nsp1 inhibits the IFN-β production by preventing TBK1 phosphorylation and inducing CBP degradation | ||
Autophagy CKLF induces microglial activation via triggering defective mitophagy and mitochondrial dysfunction |