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  • Featured Product
  • KD/KO Validated

ING2 Polyclonal antibody

ING2 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11560-1-AP

Print datasheet

Synonyms

ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2

验证数据展示

  • WB analysis of HEK-293 using 11560-1-AP

    WB analysis of HEK-293 using 11560-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11560-1-AP (ING2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 11560-1-AP

    IHC staining of human colon cancer using 11560-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ING2 antibody included
  • IHC staining of human colon cancer using 11560-1-AP

    IHC staining of human colon cancer using 11560-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11560-1-AP (ING2 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ING2 antibody included
  • IP experiment of HEK-293 using 11560-1-AP

    IP experiment of HEK-293 using 11560-1-AP

    IP result of anti-ING2 (IP:11560-1-AP, 3ug; Detection:11560-1-AP 1:500) with HEK-293 cells lysate 1000ug.

  • IF Staining of HEK-293 using 11560-1-AP

    IF Staining of HEK-293 using 11560-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HEK-293 cells using 11560-1-AP (ING2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293 cells, human liver tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ING2 Ready-To-Use IHC Kit. ING2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11560-1-AP targets ING2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ING2 fusion protein Ag2121 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name inhibitor of growth family, member 2
Synonyms ING1L, ING1Lp, ING2, Inhibitor of growth protein 2, p32, p33ING2
Calculated Molecular Weight 280 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC030128
Gene Symbol ING2
Gene ID (NCBI) 3622
RRIDAB_2127771
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H160
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ING2, also named as ING1L, belongs to the ING family and contains a PHD-type zinc finger. It is induced by the DNA-damaging agent neocarzinostatin. As a candidate tumor suppressor, ING2 seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. It is a component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. Recent finding reported that ING2 regulates muscle differentiation by regulating chormatin remodeling program. Catalog#11560-1-AP is a rabbit polyclonal antibody raised against full length ING2 of human origin.

实验方案

Product Specific Protocols
WB protocol for ING2 antibody 11560-1-APDownload protocol
IHC protocol for ING2 antibody 11560-1-APDownload protocol
IF protocol for ING2 antibody 11560-1-APDownload protocol
IP protocol for ING2 antibody 11560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Human family with sequence similarity 60 member A (FAM60A) protein: a new subunit of the Sin3 deacetylase complex.

Authors - Smith Karen T KT
View Article
humanWB

Chem Biol

Deacetylase inhibitors dissociate the histone-targeting ING2 subunit from the Sin3 complex.

Authors - Smith Karen T KT
  • KD Validated
View Article
humanWB

Cancers (Basel)

Antithetic hTERT Regulation by Androgens in Prostate Cancer Cells: hTERT Inhibition Is Mediated by the ING1 and ING2 Tumor Suppressors.

Authors - Sophie Bartsch
  • KD Validated
View Article
human,mouseWB,IHC,IF

Front Cell Dev Biol

ING2 Controls Mitochondrial Respiration via Modulating MRPL12 Ubiquitination in Renal Tubular Epithelial Cells.

Authors - Ying Yang
  • KD Validated
View Article
humanIHC

Cancer Lett

Decreased expression of ING2 gene and its clinicopathological significance in hepatocellular carcinoma.

Authors - Zhang Hua-kun HK
View Article
IP

Biochem J

Histone H3 N-terminal mimicry drives a novel network of methyl-effector interactions.

Authors - Jianji Chen
View Article