IGF2R-Specific Polyclonal antibody
IGF2R-Specific Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, L02 cells, HepG2 cells |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human liver cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20253-1-AP targets IGF2R-Specific in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, bovine |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | insulin-like growth factor 2 receptor |
Synonyms | CD222, CI Man 6 P receptor, CI MPR, CIMPR, IGF II receptor, IGF2R, IGF2R-Specific, M6P R, M6P/IGF2 receptor, M6P/IGF2R, M6PR, MPR 300, MPR1, MPRI |
Calculated Molecular Weight | 274 kDa |
Observed Molecular Weight | 300 kDa |
GenBank Accession Number | NM_000876 |
Gene Symbol | IGF2R |
Gene ID (NCBI) | 3482 |
RRID | AB_10859779 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P11717 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IHC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IF protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
IP protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
FC protocol for IGF2R-Specific antibody 20253-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Hum Mol Genet Progranulin regulates lysosomal function and biogenesis through acidification of lysosomes. | ||
J Agric Food Chem Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams. | ||
Mol Biol Cell Hydrogen peroxide induces Arl1 degradation and impairs Golgi-mediated trafficking. | ||
J Cancer Trop2 binding IGF2R induces gefitinib resistance in NSCLC by remodeling the tumor microenvironment. | ||
Life Sci Alliance Identification and characterization of a membrane receptor that binds to human STC1. | ||
J Sci Food Agric Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation. |