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IFITM1-Specific Monoclonal antibody
IFITM1-Specific Monoclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5B5E2
验证数据展示
经过测试的应用
| Positive WB detected in | TF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | K-562 cells |
| Positive FC detected in | U2OS cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
60074-1-Ig targets IFITM1-Specific in WB,IP,IHC,IF,FC,ELISA applications and shows reactivity with human samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB,IP,IHC,IF,FC |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | IFITM1-Specific fusion protein Ag2320 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | interferon induced transmembrane protein 1 (9-27) |
| Synonyms | 9 27, CD225, IFI17, IFITM1, IFITM1-Specific, Interferon induced protein 17, Leu 13 antigen, LEU13 |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-17 kDa |
| GenBank Accession Number | BC000897 |
| Gene Symbol | IFITM1 |
| Gene ID (NCBI) | 8519 |
| RRID | AB_2233405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P13164 |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IHC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IF protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IP protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| FC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise. | ||
Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
Sci Transl Med Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition. | ||
Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
| ||
