KE000010 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL5 ELISA kit is to be used to detect and quantify protein levels of endogenous IL5. The assay recognizes human IL5. A polyclonal antibody specific for IL5 has been pre-coated onto the microwells. The IL5 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL5 is added to detect the captured IL5 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.