Human CD3E Kit

Catalog number: KE00026

规格

单价

96 T

¥2600

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Overview


Product name:
Human CD3E Kit

Tests:
1 X 96 well plate

Sample type:
serum; Plasma

Assay type:
Sandwich

Sensitivity:
5 pg/mL

Range:
31.25-2000 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Plasma 94 82-107

Product overview:
KE00026 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD3E ELISA kit is to be used to detect and quantify protein levels of endogenous CD3E. The assay recognizes human CD3E. A polyclonal antibody specific for CD3E has been pre-coated onto the microwells. The CD3E protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD3E is added to detect the captured CD3E protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
CD3E, T3E, TCRE
Background

CD3E encodes the protein CD3-epsilon polypeptide, also named as FLJ18683, T3E and TCRE. CD3-epsilon is a T-cell surface glycoprotein and an essential component of the T-cell receptor (TCR) 3 complex, along with CD3-gamma, -delta and –zeta, for antigen presentation. CD3-epsilon plays a dual role within TCR3: it initiates receptor assembly within the endoplasmic reticulum and mediates signal transduction during antigen recognition, thus it is essential for T-cell development. Individuals carrying defects in this gene display immunodeficiencies such as severe combined immunodeficiency. The molecular weight of CD3E is 23kd and it is not glycosylated. The molecular weight of CD3E is 23kd and it is not glycosylated. This kit can be used to test for the presence of CD3-epsilon in vivo(serum or plasma.)


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