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- KD/KO Validated
HuR/ELAVL1 Polyclonal antibody
HuR/ELAVL1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
FC, IF/ICC, IHC, IP, WB, ELISA and More (4)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, Jurkat cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
| Positive FC detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy HuR/ELAVL1 Ready-To-Use IHC Kit. HuR/ELAVL1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11910-1-AP targets HuR/ELAVL1 in WB, IP, IF, FC, RIP, IHC, CoIP, CLIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, RIP, IHC, CoIP, CLIP, ChIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | HuR/ELAVL1 fusion protein Ag2249 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | ELAV (embryonic lethal, abnormal vision, Drosophila)-like 1 (Hu antigen R) |
| Synonyms | ELAV like protein 1, ELAV1, ELAVL1, Hu antigen R, Hua, HuR, MelG |
| Calculated Molecular Weight | 326 aa, 36 kDa |
| Observed Molecular Weight | 30-37 kDa |
| GenBank Accession Number | BC003376 |
| Gene Symbol | ELAVL1 |
| Gene ID (NCBI) | 1994 |
| RRID | AB_11182183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15717 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ELAVL1, also named as HUR, belongs to the RRM elav family. It is involved in 3'-UTR ARE-mediated MYC stabilization. ELAVL1 binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, ELAVL1 binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA and AUUUUUA motifs.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HuR/ELAVL1 antibody 11910-1-AP | Download protocol |
| IHC protocol for HuR/ELAVL1 antibody 11910-1-AP | Download protocol |
| IF protocol for HuR/ELAVL1 antibody 11910-1-AP | Download protocol |
| IP protocol for HuR/ELAVL1 antibody 11910-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cancer SULT1A1-dependent sulfonation of alkylators is a lineage-dependent vulnerability of liver cancers | ||
Nat Protoc Global analysis of RNA-binding protein dynamics by comparative and enhanced RNA interactome capture. | ||
Acta Neuropathol Nuclear depletion of RNA-binding protein ELAVL3 (HuC) in sporadic and familial amyotrophic lateral sclerosis. | ||
Mol Cell Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal. | ||
Autophagy m6A reader YTHDC1 modulates autophagy by targeting SQSTM1 in diabetic skin
| ||
Nat Commun Discovery of RNA-binding proteins and characterization of their dynamic responses by enhanced RNA interactome capture. |
