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HSP20 Polyclonal antibody

HSP20 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16813-1-AP

Print datasheet

Synonyms

Heat shock protein beta 6, Hsp20, HSPB6

验证数据展示

  • WB analysis using 16813-1-AP

    WB analysis using 16813-1-AP

    rat heart tissues were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16813-1-AP

    IHC staining of mouse heart using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPB6 antibody included
  • IF Staining of mouse heart using 16813-1-AP

    IF Staining of mouse heart using 16813-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using HSP20 antibody (16813-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 16813-1-AP

    WB analysis using 16813-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16813-1-AP (HSP20 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 16813-1-AP

    IHC staining of mouse heart using 16813-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16813-1-AP (HSP20 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSPB6 antibody included

经过测试的应用

Positive WB detected inrat heart tissue, mouse heart tissue
Positive IHC detected inmouse heart tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy HSPB6 Ready-To-Use IHC Kit. HSPB6 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16813-1-AP targets HSP20 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Tested Reactivity human, rat, mouse
Immunogen HSP20 fusion protein Ag10352 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein, alpha-crystallin-related, B6
Synonyms Heat shock protein beta 6, Hsp20, HSPB6
Calculated Molecular Weight 160 aa, 17 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC068046
Gene Symbol HSPB6
Gene ID (NCBI) 126393
RRIDAB_2878321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14558
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function.

实验方案

Product Specific Protocols
WB protocol for HSP20 antibody 16813-1-APDownload protocol
IHC protocol for HSP20 antibody 16813-1-APDownload protocol
IF protocol for HSP20 antibody 16813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols