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HSP90 Polyclonal antibody

HSP90 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

FC, IHC, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 13171-1-AP

Print datasheet

Synonyms

Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, PC-12 cells, C6 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inK-562 cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells, A549 cells
Planning an IHC experiment? We recommend our IHCeasy HSP90AA1 Ready-To-Use IHC Kit. HSP90AA1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13171-1-AP targets HSP90 in WB, RIP, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IHC, IP, WB, ELISA
Cited Applications CoIP, ELISA, IF, IHC, IP, RIP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken, carp, fish, pig, bovine
Immunogen HSP90 fusion protein Ag3826 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name heat shock protein 90kDa alpha (cytosolic), class A member 1
Synonyms Heat shock 86 kDa, HSP 86, HSP86, Hsp89, HSP89A, HSP90, HSP90A, HSP90AA1, HSP90N, HSPC1, HSPCA, HSPCAL1, HSPCAL4, HSPN, LAP2
Calculated Molecular Weight 853 aa, 90 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC023006
Gene Symbol HSP90AA1
Gene ID (NCBI) 3320
RRIDAB_2120924
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07900
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP90, encoded by HSP90AA1, is a constitutively and ubiquitously expressed molecular chaperone that is crucial for the stability and function of many proteins. HSP90 provides chaperoning activity for client proteins; many of them are members of oncogenic pathways, indicating its implication in tumor malignancy. HSP90 mainly resides in the cytosol, while it can also be released to the extracellular space. Secreted Hsp90 is a C-terminal truncated form. It has been reported that the level of plasma Hsp90 is positively correlated with tumor malignancy in clinical cancer patients, and can be a promising diagnostic marker for tumor malignancy in clinical application.

实验方案

Product Specific Protocols
WB protocol for HSP90 antibody 13171-1-APDownload protocol
IHC protocol for HSP90 antibody 13171-1-APDownload protocol
IF protocol for HSP90 antibody 13171-1-APDownload protocol
IP protocol for HSP90 antibody 13171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.

Authors - Lingyun Long
CoIP

Nat Cell Biol

LIMIT is an immunogenic lncRNA in cancer immunity and immunotherapy.

Authors - Gaopeng Li
mouseWB

Nat Commun

FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment

Authors - Zongchao Sun
mouseWB

Nat Commun

The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells

Authors - Xiaofeng Liao
humanWB

Mol Cell

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

Authors - Gayathri Muthukumar

Mol Cell

SENP1-Sirt3 Signaling Controls Mitochondrial Protein Acetylation and Metabolism.

Authors - Tianshi Wang