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HLA-F Monoclonal antibody

HLA-F Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B12C2

Cat No : 66819-1-Ig

Print datasheet

Synonyms

CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F

验证数据展示

  • WB analysis using 66819-1-Ig

    WB analysis using 66819-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66819-1-Ig (HLA-F antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 66819-1-Ig

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 66819-1-Ig

    IHC staining of human tonsillitis using 66819-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66819-1-Ig (HLA-F antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of Raji using 66819-1-Ig

    FC experiment of Raji using 66819-1-Ig

    1x10^6 Raji cells were intracellularly stained with 0.4 ug Anti-Human HLA-F (66819-1-Ig, Clone:1B12C2) (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inA431 cells, NCCIT cells, Raji cells, human peripheral blood leukocytes
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inRaji cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:8000-1:32000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66819-1-Ig targets HLA-F in WB, IHC, FC, ELISA applications and shows reactivity with Human samples.

Tested Applications FC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen HLA-F fusion protein Ag6475 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name major histocompatibility complex, class I, F
Synonyms CDA12, HLA 5.4, HLA CDA12, HLA F, HLA F antigen, HLAF, HLA-F, Leukocyte antigen F, MHC class I antigen F
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 40-45 kDa
GenBank Accession NumberBC062991
Gene Symbol HLA-F
Gene ID (NCBI) 3134
RRIDAB_2882162
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP30511
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human major histocompatibility complex (MHC) antigens, also referred to as human leukocyte antigens (HLA), are encoded by genes located on the short arm of chromosome 6 (6p21.3). There are two classes of HLA antigens: class I and class II. This class I molecules are membrane glycoproteins composed of a heavy (alpha) chain which is encoded by a HLA class I gene, and β2-microglobulin light (beta) chain. The most extensively characterized members of the HLA class I gene family are the genes encoding the major transplantation antigenes, HLA-A, B and C. HLA-F is a non-classical MHC class I molecule. (PMID: 667938; 3375250; 2249951)

实验方案

Product Specific Protocols
WB protocol for HLA-F antibody 66819-1-IgDownload protocol
IHC protocol for HLA-F antibody 66819-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols