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CoraLite®594-conjugated Glutamine Synthetase Monoclonal antibody

Glutamine Synthetase Monoclonal Antibody for FC (Intra), IF

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1B6G4

Cat No : CL594-66323

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Synonyms

GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59



经过测试的应用

Positive IF detected inmouse brain tissue
Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66323-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66323 targets Glutamine Synthetase in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF
Tested Reactivity Human, Mouse, Rat
Immunogen Glutamine Synthetase fusion protein Ag6309 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamate-ammonia ligase (glutamine synthetase)
Synonyms GLNS, GLUL, Glutamate ammonia ligase, Glutamate decarboxylase, Glutamine synthetase, GS, PIG43, PIG59
Calculated Molecular Weight 374 aa, 42 kDa
GenBank Accession NumberBC011700
Gene Symbol GLUL
Gene ID (NCBI) 2752
RRIDAB_2883558
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP15104
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLUL(Glutamine synthetase) is also named as GS,GLNS and belongs to the glutamine synthetase family.This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner By similarity. Essential for proliferation of fetal skin fibroblasts(PMID:18662667).Defects in GLUL are the cause of congenital systemic glutamine deficiency (CSGD).Organismal glutamine production is augmented secondary to an increase in the activity of glutamine synthetase in the lung and skeletal muscle(PMID:7630137). The antibody is conjugated with CL594, Ex/Em 593 nm/614 nm.

实验方案

Product Specific Protocols
IF protocol for CL594 Glutamine Synthetase antibody CL594-66323Download protocol
Standard Protocols
Click here to view our Standard Protocols