GTF3C2 Polyclonal antibody

GTF3C2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27494-1-AP

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Synonyms

GTF3C2, KIAA0011, TF3C beta, TFIIIC 110 kDa subunit, TFIIIC BETA, TFIIIC110



经过测试的应用

Positive WB detected inHEK-293T cells, A549 cells, HeLa cells, HepG2 cells, MCF-7 cells, PC-3 cells
Positive IHC detected inhuman thyroid cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy GTF3C2 Ready-To-Use IHC Kit. GTF3C2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27494-1-AP targets GTF3C2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human
Immunogen GTF3C2 fusion protein Ag26666 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name general transcription factor IIIC, polypeptide 2, beta 110kDa
Synonyms GTF3C2, KIAA0011, TF3C beta, TFIIIC 110 kDa subunit, TFIIIC BETA, TFIIIC110
Calculated Molecular Weight 101 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC020981
Gene Symbol GTF3C2
Gene ID (NCBI) 2976
RRIDAB_2880889
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WUA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GTF3C2, also named as KIAA0011, is a 911 amino acid protein, which localizes in the nucleus. GTF3C2 is required for RNA polymerase III-mediated transcription. GTF3C2 is a component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. GTF3C2 may play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.

实验方案

Product Specific Protocols
WB protocol for GTF3C2 antibody 27494-1-APDownload protocol
IHC protocol for GTF3C2 antibody 27494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols