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GPR3 Polyclonal antibody

GPR3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Conjugate

Unconjugated

Cat No : 20847-1-AP

Print datasheet

Synonyms

ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3

验证数据展示

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 20847-1-AP

    IHC staining of mouse brain using 20847-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20847-1-AP (GPR3 antibody) at dilution of 1:50 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20847-1-AP targets GPR3 in IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, ELISA
Tested Reactivity human, mouse
Immunogen GPR3 fusion protein Ag14257 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name G protein-coupled receptor 3
Synonyms ACCA, ACCA orphan receptor, G protein coupled receptor 3, GPR3
Calculated Molecular Weight 330 aa, 35 kDa
GenBank Accession NumberBC032702
Gene Symbol GPR3
Gene ID (NCBI) 2827
RRIDAB_2878749
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP46089
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR3 (G protein-coupled receptor 3) was first cloned from a mouse cDNA library in 1993 before being cloned from human genomic libraries by several independent groups (PMID: 29941868). GPR3 mRNA is broadly expressed in neurons in various brain regions, including the cortex, thalamus, hypothalamus, amygdala, hippocampus, pituitary, and cerebellum (PMID: 22207171). Moreover, the GPR3 protein is overexpressed in neurons in post-mortem brain tissue sections from individuals afflicted by Alzheimer's disease (PMID: 19213921).

实验方案

Product Specific Protocols
IHC protocol for GPR3 antibody 20847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols