GPNMB Monoclonal antibody

GPNMB Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

IF, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2B10B8

Cat No : 66926-1-Ig

Print datasheet

Synonyms

GPNMB, HGFIN, Transmembrane glycoprotein NMB



经过测试的应用

Positive WB detected inSK-BR-3 cells, HT-1080 cells, HeLa cells, A375 cells, U-251 cells, THP-1 cells, RAW 264.7 cells, PC-12 cells, U-937 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66926-1-Ig targets GPNMB in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited ApplicationsIF, IHC, WB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen GPNMB fusion protein Ag26747 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glycoprotein (transmembrane) nmb
Synonyms GPNMB, HGFIN, Transmembrane glycoprotein NMB
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64-70 kDa
GenBank Accession NumberBC011595
Gene Symbol GPNMB
Gene ID (NCBI) 10457
RRIDAB_2882253
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14956
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPNMB also known as HGFIN, osteoactivin, and DC-HIL, is a type I membrane glycoprotein involved in various biological processes, including inflammation, invasion and metastasis of malignant tumors, cell differentiation, and tissue regeneration. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB acts as an osteogenic factor that stimulates osteoblast differentiation in vivo and in vitro.

实验方案

Product Specific Protocols
WB protocol for GPNMB antibody 66926-1-IgDownload protocol
IHC protocol for GPNMB antibody 66926-1-IgDownload protocol
IF protocol for GPNMB antibody 66926-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC,IF

Int Immunopharmacol

Lactic acid-induced M2-like macrophages facilitate tumor cell migration and invasion via the GPNMB/CD44 axis in oral squamous cell carcinoma

Authors - Ying Lin
mouseWB,IF

Int Immunopharmacol

PCSK9 inhibitor protects against ischemic cerebral injury by attenuating inflammation via the GPNMB/CD44 pathway

Authors - Yaling Zheng
human,mouseWB,IHC

Cell Signal

A comprehensive evaluation of circ_0065214/ miR-188-3p/GPNMB axis in breast cancer

Authors - Xi-Xi Wu
humanIHC

Cancers (Basel)

TROP-2, Nectin-4, GPNMB, and B7-H3 Are Potentially Therapeutic Targets for Anaplastic Thyroid Carcinoma

Authors - Soji Toda