• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

GP73/GOLPH2 Polyclonal antibody

GP73/GOLPH2 Polyclonal Antibody for FC, IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

FC, IF/ICC, IP, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 15126-1-AP

Print datasheet

Synonyms

golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, golgi protein 73kD, GOLM1, GOLPH2, GP73, GP73/GOLPH2

验证数据展示

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15126-1-AP (GP73/GOLPH2 antibody), at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). The cytoskeleton and nuclei were stained with CL594-phalloidin and DAPI, respectively.

  • WB analysis using 15126-1-AP

    WB analysis using 15126-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 15126-1-AP

    IP experiment of HeLa using 15126-1-AP

    IP result of anti-GP73/GOLPH2 (IP:15126-1-AP, 4ug; Detection:15126-1-AP 1:1000) with HeLa cells lysate 2800ug.

  • FC experiment of HeLa using 15126-1-AP

    FC experiment of HeLa using 15126-1-AP

    1X10^6 HeLa cells were stained with 0.2ug GP73/GOLPH2 antibody (15126-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IHC staining of human liver cancer using 15126-1-AP

    IHC staining of human liver cancer using 15126-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15126-1-AP (GP73/GOLPH2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with GOLM1 antibody included
  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 15126-1-AP

    IF Staining of HeLa using 15126-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GP73/GOLPH2 antibody (15126-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inLNCaP cells, HuH-7 cells, MDA-MB-231 cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15126-1-AP targets GP73/GOLPH2 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications FC, IF/ICC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen GP73/GOLPH2 fusion protein Ag7207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi membrane protein 1
Synonyms golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2, golgi protein 73kD, GOLM1, GOLPH2, GP73, GP73/GOLPH2
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC001740
Gene Symbol GOLM1
Gene ID (NCBI) 51280
RRIDAB_2113330
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NBJ4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 70-75 kDa in western blotting due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IHC protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IF protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
IP protocol for GP73/GOLPH2 antibody 15126-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS Pathog

GP73 represses host innate immune response to promote virus replication by facilitating MAVS and TRAF6 degradation.

Authors - Xuewu Zhang
  • KD Validated
View Article
humanWB

J Cell Sci

Rapid affinity purification of intracellular organelles using a twin strep tag.

Authors - Jian Xiong
View Article
humanIF

Chemistry

Investigation of Glycosylphosphatidylinositol (GPI)-Plasma Membrane Interaction in Live Cells and the Influence of GPI Glycan Structure on the Interaction

Authors - Sayan Kundu
View Article
humanWB,IHC,IF

J Cancer

GP73 level determines chemotherapeutic resistance in human hepatocellular carcinoma cells.

Authors - Jia-Zhou Ye
  • KD Validated
View Article

Curr Alzheimer Res

RCAN1 Increases Aβ Generation by Promoting N-Glycosylation Via Oligosaccharyl Transferase.

Authors - T Wang
View Article
humanWB

Eur Rev Med Pharmacol Sci

LncRNA MALAT1 knockdown inhibits cell migration and invasion by suppressing autophagy through miR-384/GOLM1 axis in glioma.

Authors - R Ma
View Article