• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

GLUD2 Polyclonal antibody

GLUD2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 14462-1-AP

Print datasheet

Synonyms

GDH 2, GDH2, GLUD2, GLUDP1, glutamate dehydrogenase 2

验证数据展示

  • WB analysis of HepG2 using 14462-1-AP

    WB analysis of HepG2 using 14462-1-AP

    WB result of GLUD2 antibody (14462-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GLUD2 transfected HepG2 cells.

  • WB analysis using 14462-1-AP

    WB analysis using 14462-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14462-1-AP (GLUD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14462-1-AP

    IHC staining of human prostate cancer using 14462-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human prostate cancer using 14462-1-AP

    IHC staining of human prostate cancer using 14462-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14462-1-AP (GLUD2 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 14462-1-AP

    IF Staining of HepG2 using 14462-1-AP

    Immunofluorescent analysis of HepG2 cells, using GLUD2 antibody 14462-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

经过测试的应用

Positive WB detected inmouse eye tissue, HepG2 cells, rat testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14462-1-AP targets GLUD2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited Applications IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen GLUD2 fusion protein Ag5748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate dehydrogenase 2
Synonyms GDH 2, GDH2, GLUD2, GLUDP1, glutamate dehydrogenase 2
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC050732
Gene Symbol GLUD2
Gene ID (NCBI) 2747
RRIDAB_2110642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49448
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GLUD2 antibody 14462-1-APDownload protocol
IHC protocol for GLUD2 antibody 14462-1-APDownload protocol
IF protocol for GLUD2 antibody 14462-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Metab

Gut microbiome-derived ammonia modulates stress vulnerability in the host

Authors - Pei Wang
View Article
humanIHC

Transl Oncol

Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.

Authors - Xuyong Wei
View Article
humanWB

Amino Acids

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Authors - Chunhong Cui
View Article