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  • KD/KO Validated

KGA/GAM/GAC Polyclonal antibody

KGA/GAM/GAC Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 23549-1-AP

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Synonyms

AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase



经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells, HeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:25-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 23549-1-AP is KGA/GAM/GAC Fusion Protein expressed in E. coli.

Tested Applications IF, IHC, IP, WB, ELISA
Cited ApplicationsIF, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KGA/GAM/GAC fusion protein Ag20354 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutaminase
Synonyms AAD20, GAC, GAM, GLS, GLS1, glutaminase, K glutaminase, KGA, KGA/GAM/GAC, KIAA0838, L glutamine amidohydrolase
Calculated Molecular Weight 669 aa, 73 kDa
Observed Molecular Weight 58 kDa, 65 kDa, 83 kDa
GenBank Accession NumberBC038507
Gene Symbol GLS
Gene ID (NCBI) 2744
RRIDAB_2879295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94925
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GLS, also named as GLS1 and KIAA0838, belongs to the glutaminase family. It catalyzes the first reaction in the primary pathway for the renal catabolism of glutamine. Glutaminase-, glutamate-, and taurine-immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease.The glutaminase band in AA/C1 cells is more intense than in HT29 cells, in accordance with measurements of glutaminase activity, and had the same molecular mass of approx. 63 kDa(PMID:12408749). Ping Gao et al. (2009) determined that mitochondrial glutaminase expression (GLS, molecular mass of ~58 kDa) is increased ~10-fold in response to Myc. It also reveals a molecular weight of 83-84 kDa as a phosphate-dependent glutaminase(PMID:447624;7512428). GLS has 3 isoforms produced by alternative splicing and this antibody can recognize all the 3 isoforms(KGA,GAM,GAC) of GLS.

实验方案

Product Specific Protocols
WB protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IHC protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IF protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
IP protocol for KGA/GAM/GAC antibody 23549-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
  • KO Validated
humanWB

Redox Biol

Mitochondrial thiol modification by a targeted electrophile inhibits metabolism in breast adenocarcinoma cells by inhibiting enzyme activity and protein levels.

Authors - M Ryan Smith