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GCLM Monoclonal antibody

GCLM Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse and More (1)

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A5A4

Cat No : 66808-1-Ig

Print datasheet

Synonyms

Gamma ECS regulatory subunit, GCLM, GCS light chain, GLCLR



经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, RAW 264.7 cells
Positive IHC detected inhuman liver cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

66808-1-Ig targets GCLM in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity Human, rat, mouse
Cited Reactivityhuman, rat
Immunogen GCLM fusion protein Ag5497 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glutamate-cysteine ligase, modifier subunit
Synonyms Gamma ECS regulatory subunit, GCLM, GCS light chain, GLCLR
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC041809
Gene Symbol GCLM
Gene ID (NCBI) 2730
RRIDAB_2882151
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48507
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for GCLM antibody 66808-1-IgDownload protocol
IHC protocol for GCLM antibody 66808-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oxid Med Cell Longev

Farrerol Enhances Nrf2-Mediated Defense Mechanisms against Hydrogen Peroxide-Induced Oxidative Damage in Human Retinal Pigment Epithelial Cells by Activating Akt and MAPK.

Authors - Ning Ma
ratWB

Eur J Med Chem

Design, synthesis and evaluation of fused hybrids with acetylcholinesterase inhibiting and Nrf2 activating functions for Alzheimer's disease

Authors - Yuanyuan Wang
humanWB

Front Cell Dev Biol

Single-cell and genetic multi-omics analysis combined with experiments confirmed the signature and potential targets of cuproptosis in hepatocellular carcinoma

Authors - Feng Cao