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G6PD Polyclonal antibody
G6PD Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
IF, IHC, WB,ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, HeLa cells, HEK-293 cells |
Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 25413-1-AP is G6PD Fusion Protein expressed in E. coli.
Tested Applications | IF, IHC, WB,ELISA |
Cited Applications | CoIP, IF, IHC, IP, WB |
Tested Reactivity | human |
Cited Reactivity | human, mouse, rat, sheep |
Immunogen | G6PD fusion protein Ag21862 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | glucose-6-phosphate dehydrogenase |
Synonyms | G6PD, G6PD1 |
Calculated Molecular Weight | 59 kDa |
Observed Molecular Weight | 59 kDa |
GenBank Accession Number | BC000337 |
Gene Symbol | G6PD |
Gene ID (NCBI) | 2539 |
RRID | AB_2880066 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P11413 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for G6PD antibody 25413-1-AP | Download protocol |
IHC protocol for G6PD antibody 25413-1-AP | Download protocol |
IF protocol for G6PD antibody 25413-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Sci Adv G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis. | ||
Nat Commun Polo-like kinase 1 coordinates biosynthesis during cell cycle progression by directly activating pentose phosphate pathway. | ||
Acta Pharmacol Sin Targeting proteasomal deubiquitinases USP14 and UCHL5 with b-AP15 reduces 5-fluorouracil resistance in colorectal cancer cells | ||
Phytomedicine Tiliroside targets TBK1 to induce ferroptosis and sensitize hepatocellular carcinoma to sorafenib | ||
Oxid Med Cell Longev Cigarette Smoke Exposure Increases Glucose-6-phosphate Dehydrogenase, Autophagy, Fibrosis, and Senescence in Kidney Cells In Vitro and In Vivo. | ||
Apoptosis Role of Escin in breast cancer therapy: potential mechanism for inducing ferroptosis and synergistic antitumor activity with cisplatin
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